P-37: Evaluating Orally Administration of Aquatic Extract of Saffron (Crocus Sativus L.) on Histomorphometric Changes of Testicular Tissue in Stress Induced Rats

Background: Saffron consists of dried stigmas and top of the styles of crocus sativus. It has various applications such as sexual potential stimulant, anti spasm, anti depression, sedative ,anti inflammation and anti cancer. Materials and Methods: In present study, 200 male adult rats divided to five groups with 10 rats per each cage and with four replicate for each group. Solvent of saffron was prepared by soaking 1 g of saffron in 40 ml of distilled water. The groups followed of: 1] control group (Normal saline 10 ml/kg, orally); 2] saffron (50 mg/kg, orally); 3] saffron (100 mg/kg, orally); 4] saffron (200 mg/kg, orally); 5] saffron (200mg/kg orally with excessive noise stress). For stress induction, a cassette player had playing excessive noise were used in front of cage by 0/5 meter distance with it. The testis tissue was sampled after passing of above time and section providing, was stained by H and E and then was observed by microscope. Results: The seminiferous tubules diameter, seminiferous epithelial thickness and testicular capsule thickness significantly decreased after saffron administration compared with control group and this decreasing is more significant in stress condition. Interstitial tissue increased after saffron administration compared with control group and this increasing is more significant. Conclusion: Our results showed seminiferous tubules diameter, seminiferous epithelial thickness, interstitial tissue and testicular capsule thickness were significantly changed after saffron orally administration in stress condition. These results can cause by reducing of serum testosterone concentration. It suggested the effect of saffron on histomorphometric changes at stress condition in human can be more investigation.